{"id":1389,"date":"2023-12-27T01:13:26","date_gmt":"2023-12-26T17:13:26","guid":{"rendered":"http:\/\/baijianyy.cn\/?p=1389"},"modified":"2023-12-27T01:13:26","modified_gmt":"2023-12-26T17:13:26","slug":"%e9%b9%b0%e6%bd%ad%e5%b8%82%e8%b4%b5%e6%ba%aa%e5%b8%82%e9%98%bb%e6%96%ad%e8%8d%af%e5%93%aa%e9%87%8c%e6%9c%89%e5%8d%96%e7%9a%84%ef%bc%9f%e8%8e%b7%e5%be%97%e8%af%ba%e8%b4%9d%e5%b0%94%e5%a5%96%e7%9a%84cr","status":"publish","type":"post","link":"http:\/\/baijianyy.cn\/?p=1389","title":{"rendered":"\u9e70\u6f6d\u5e02\u8d35\u6eaa\u5e02\u963b\u65ad\u836f\u54ea\u91cc\u6709\u5356\u7684\uff1f\u83b7\u5f97\u8bfa\u8d1d\u5c14\u5956\u7684CRISPR\u6280\u672f\u5bf9\u6cbb\u6108\u827e\u6ecb\u75c5\u53d6\u5f97\u91cd\u5927\u5b9e\u9a8c\u7a81\u7834"},"content":{"rendered":"<p>\u827e\u6ecb\u75c5<a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u963b\u65ad\u836f\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e9%98%bb%e6%96%ad%e8%8d%af\" target=\"_blank\" rel=\"noopener\">\u963b\u65ad\u836f<\/a>\u54ea\u91cc\u8d2d\u4e70\uff0c\u827e\u6ecb\u75c5<a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u963b\u65ad\u836f\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e9%98%bb%e6%96%ad%e8%8d%af\" target=\"_blank\" rel=\"noopener\">\u963b\u65ad\u836f<\/a>\u4ef7\u683c\uff0c\u827e\u6ecb\u75c5<a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u963b\u65ad\u836f\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e9%98%bb%e6%96%ad%e8%8d%af\" target=\"_blank\" rel=\"noopener\">\u963b\u65ad\u836f<\/a>\u54c1\u9500\u552e\u673a\u6784\uff1f<br \/>\npep<a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u963b\u65ad\u836f\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e9%98%bb%e6%96%ad%e8%8d%af\" target=\"_blank\" rel=\"noopener\">\u963b\u65ad\u836f<\/a>\u3001prep<a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u963b\u65ad\u836f\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e9%98%bb%e6%96%ad%e8%8d%af\" target=\"_blank\" rel=\"noopener\">\u963b\u65ad\u836f<\/a>\u5168\u56fd\u4e24\u5c0f\u65f6\u6025\u9001\uff0c\u836f\u5e97\u76f4\u53d1\uff0c\u4e13\u4eba\u4e13\u9001<\/p>\n<p><a class=\"tag_link\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201c\u827e\u6ecb\u75c5\u201d\u7684\u6587\u7ae0\" href=\"http:\/\/baijianyy.cn\/?tag=%e8%89%be%e6%bb%8b%e7%97%85\" target=\"_blank\" rel=\"noopener\">\u827e\u6ecb\u75c5<\/a>HIV\u66b4\u9732\u524d\u540e\u963b\u65ad\u9884\u9632\u7528\u836f\uff0c\u8bf7\u626b\u7801\u6dfb\u52a0\u533b\u9662\u5ba2\u670d\uff0c<strong>\u5168\u56fd\u5404\u57ce\u5e02<\/strong>\u6700\u5feb2\u5c0f\u65f6\u5de6\u53f3\u9001\u8fbe\u3002<\/p>\n<p>\u9ad8\u5371\u884c\u4e3a\u540e\u591a\u4e45\u68c0\u6d4b\uff1f\u9ad8\u5371\u6027\u884c\u4e3a\u4e00\u6b21\u8981\u5403\u963b\u65ad\u836f\u5417\uff1f\u53d1\u751f\u9ad8\u5371\u6027\u884c\u4e3a\u540e\u600e\u4e48\u529e\uff1f\u9ad8\u5371\u884c\u4e3a\u591a\u5c11\u5929\u540e\u53ef\u4ee5\u5f7b\u5e95\u6392\u9664\uff1f\u7b49\u7b49\u95ee\u9898\uff0c\u53ef\u4ee5\u5728\u4e92\u8054\u7f51\u533b\u9662\u514d\u8d39\u54a8\u8be2\u4e13\u4e1a\u611f\u67d3\u79d1\u533b\u5e08\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-127 aligncenter\" src=\"http:\/\/baijianyy.cn\/wp-content\/uploads\/2023\/12\/mmexport1702183130138.gif\" alt=\"\" width=\"300\" height=\"300\" \/><\/p>\n<p>\u9e70\u6f6d\u5e02\u8d35\u6eaa\u5e02\u81ea\u613f\u54a8\u8be2\u68c0\u6d4b\u673a\u6784\uff1a\u8d35\u6eaa\u5e02\u75be\u75c5\u9884\u9632\u63a7\u5236\u4e2d\u5fc3<\/p>\n<p>\u5730\u5740\uff1a\u9e70\u6f6d\u5e02\u8d35\u6eaa\u5e02\u5efa\u8bbe\u8def350\u53f7<\/p>\n<p>\u963b\u65ad\u836f\u54ea\u91cc\u6709\u5356\u7684\uff1f\u6b64\u95ee\u9898\u8bf7\u626b\u4e0a\u9762\u7684\u7801\u5173\u6ce8\u4e92\u8054\u7f51\u533b\u9662\uff0c\u4e3a\u60a8\u89e3\u7b54<\/p>\n<hr \/>\n<p>\u5317\u4eac\u65f6\u95f42020\u5e7410\u67087\u65e5\uff0c\u8bfa\u8d1d\u5c14\u57fa\u91d1\u4f1a\u5ba3\u5e03\uff0c\u8fd1\u5e74\u6765\u706b\u70ed\u7684CRISPR\u57fa\u56e0\u7f16\u8f91\u7cfb\u7edf\u65a9\u83b7\u672c\u5e74\u5ea6\u7684\u8bfa\u8d1d\u5c14\u5316\u5b66\u5956\uff0c\u5728\u8fd9\u4e00\u9886\u57df\u505a\u51fa\u5353\u8d8a\u8d21\u732e\u7684Emmanuelle Charpentier\u6559\u6388\u548cJennifer Doudna\u6559\u6388\u6458\u5f97\u6842\u51a0\u3002<\/p>\n<p>2012\u5e748\u670817\u65e5\uff0c\u8a79\u59ae\u5f17\u00b7\u675c\u5fb7\u5a1c\uff08Jennifer 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\u6742\u5fd7\u53d1\u8868\u8bba\u6587\uff0c\u9996\u6b21\u5c06CRISPR\/Cas9\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u6539\u8fdb\u5e76\u5e94\u7528\u4e8e\u54fa\u4e73\u52a8\u7269\u548c\u4eba\u7c7b\u7ec6\u80de\u3002<\/p>\n<p>\u90a3\u4e48CRISPR\u5230\u5e95\u662f\u4ec0\u4e48\u5462\uff1f<\/p>\n<p>\u4eba\u7c7b\u8eab\u4f53\u91cc\u7684\u57fa\u56e0\uff0c\u662f\u751f\u547d\u6700\u521d\u59cb\u7684\u5bc6\u7801\uff0c\u5b83\u64cd\u7eb5\u7740\u6211\u4eec\u7684\u4e00\u5207\u3002\u57fa\u56e0\u662f\u7531\u78b1\u57fa\u5bf9\u4e24\u4e24\u76f8\u914d\u800c\u6210\u3002\u800cCRISPR\u5c31\u50cf\u662f\u4e00\u628a\u526a\u5200\uff0c\u53ef\u4ee5\u7cbe\u51c6\u5730\u526a\u5207\u67d0\u4e00\u4e2a\u78b1\u57fa\u5bf9\u3002\u4f5c\u4e3a\u57fa\u56e0\u7f16\u8f91\u7684\u5de5\u5177\uff0c\u5b83\u53ef\u4ee5\u7cbe\u51c6\u5730\u526a\u5207\u4efb\u4f55\u57fa\u56e0\u7247\u6bb5\uff0c\u5229\u7528DNA\u4fee\u590d\u7684\u539f\u7406\uff0c\u6765\u5b8c\u6210DNA\u7247\u6bb5\u7f16\u8f91\u7684\u540c\u65f6\u8ba9\u7ec6\u80de\u7ee7\u7eed\u6d3b\u4e0b\u53bb\u3002<\/p>\n<p>\u90a3\u4e48CRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u548c\u827e\u6ecb\u75c5\u7684\u6cbb\u7597\u6709\u4f55\u79cd\u8054\u7cfb\u5462\uff1f<\/p>\n<p>\u76ee\u524d\uff0c\u827e\u6ecb\u75c5\u7684\u6cbb\u7597\u65b9\u6cd5\u5c31\u662f\u6297\u9006\u8f6c\u5f55\u75c5\u6bd2\u7597\u6cd5\uff08ART\uff09\u3002\u8fd9\u79cd\u7597\u6cd5\u53ef\u4ee5\u6291\u5236\u75c5\u6bd2\u590d\u5236\u5e76\u4e14\u963b\u6b62\u75c5\u6bd2\u611f\u67d3\u8005\u8fdb\u4e00\u6b65\u6f14\u53d8\u6210\u827e\u6ecb\u75c5\u3002\u6839\u636e\u4e16\u754c\u536b\u751f\u7ec4\u7ec72017\u5e74\u6570\u636e\uff0c\u5728\u5168\u74033690\u4e07<a href=\"http:\/\/baijianyy.cn\/?tag=hiv%e6%84%9f%e6%9f%93\" title=\"\u6d4f\u89c8\u5173\u4e8e\u201cHIV\u611f\u67d3\u201d\u7684\u6587\u7ae0\" target=\"_blank\" class=\"tag_link\">HIV\u611f\u67d3<\/a>\u8005\u4e2d\uff0c\u67092170\u4e07\u4eba\u6b63\u5728\u63a5\u53d7\u6297\u9006\u8f6c\u5f55\u75c5\u6bd2\u6cbb\u7597\u3002\u4f46\u662f\uff0c\u8be5\u7597\u6cd5\u5e76\u6ca1\u6709\u6d88\u9664\u4f53\u5185\u7684\u75c5\u6bd2\uff0c\u5982\u679c\u611f\u67d3\u8005\u505c\u6b62\u6cbb\u7597\uff0c\u75c5\u6bd2\u8fd8\u4f1a\u7ee7\u7eed\u590d\u5236\uff0c\u5176\u6c34\u5e73\u53ef\u4ee5\u5728\u51e0\u5468\u65f6\u95f4\u5185\u4e0a\u5347\u3002\u800cHIV\u4e4b\u6240\u4ee5\u6709\u201c\u53cd\u5f39\u201d\u80fd\u529b\uff0c\u662f\u56e0\u4e3a\u75c5\u6bd2\u5728\u653b\u51fb\u4eba\u4f53\u7684\u514d\u75ab\u7cfb\u7edf\u65f6\uff0c\u4f1a\u5c06DNA\u5e8f\u5217\u6574\u5408\u5230\u7ec6\u80de\u7684\u57fa\u56e0\u7ec4\u4e2d\uff0c\u4ece\u800c\u85cf\u533f\u4e8e\u4eba\u4f53\u3002\u8981\u771f\u6b63\u6d88\u9664HIV\u8fbe\u5230\u201c\u6cbb\u6108\u201d\u6548\u679c\uff0c\u5c31\u8981\u4ece\u611f\u67d3\u7684\u7ec6\u80de\u548c\u7ec4\u7ec7\u4e2d\u53bb\u9664\u88ab\u75c5\u6bd2\u6574\u5408\u8fdb\u53bb\u7684DNA\u7247\u6bb5\u3002<\/p>\n<p>2019\u5e747\u67082\u65e5\uff0c\u4e00\u9879\u53d1\u8868\u5728\u6742\u5fd7\u300a\u81ea\u7136\u00b7\u901a\u8baf\u300b\uff08NatureCommunications\uff09\u4e0a\u7684\u7814\u7a76\u79f0\uff0c\u9996\u6b21\u6210\u529f\u6d88\u706d\u4e86\u6d3b\u4f53\u4eba\u6e90\u5316\u5c0f\u9f20\u4f53\u5185\u7684HIV\uff0c\u8ddd\u79bb\u627e\u5230\u53ef\u80fd\u6cbb\u6108\u827e\u6ecb\u75c5\u7684\u65b9\u6cd5\u53c8\u8fdb\u4e86\u4e00\u6b65\u3002<\/p>\n<p>\u7f8e\u56fd\u5185\u5e03\u62c9\u65af\u52a0\u5927\u5b66\u533b\u5b66\u4e2d\u5fc3\uff08Universityof Nebraska Medical Center\uff09\u548c\u5766\u666e\u5c14\u5927\u5b66\u5218\u6613\u65af\u00b7\u5361\u8328\u533b\u5b66\u9662\uff08Lewis Katz School of Medicine at Temple University\uff09\u5171\u540c\u5f00\u53d1\u4e86\u4e00\u79cd\u65e8\u5728\u6d88\u9664HIV\u75c5\u6bd2\u7684\u8054\u5408\u7597\u6cd5\uff0c\u7531\u4e00\u79cdART\u9012\u9001\u65b0\u65b9\u6cd5\u548cCRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u7ec4\u6210\u3002\u4ed6\u4eec\u80fd\u591f\u5728\u4eba\u6e90\u5316\u5c0f\u9f20\u8eab\u4e0a\u6467\u6bc1HIV\u3002\u4ed6\u4eec\u4f7f\u7528\u7684\u4eba\u6e90\u5316\u5c0f\u9f20\u6ce8\u5c04\u4e86\u4eba\u7c7b\u9aa8\u9ad3\uff0c\u4ee5\u6a21\u4eff\u4eba\u4f53\u514d\u75ab\u7cfb\u7edf\uff0c\u5f53\u7136\u8fd8\u6709HIV\u611f\u67d3\u7684\u4eba\u4f53\u514d\u75ab\u7ec6\u80de\u3002\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u8bbe\u7f6e\u4e86\u4e09\u4e2a\u72ec\u7acb\u7684\u8bd5\u9a8c\uff0c\u5e76\u5206\u522b\u7ed9\u611f\u67d3HIV\u7684\u5c0f\u9f20\u4e09\u79cd\u7597\u6cd5\uff1a\u201c\u8d85\u7ea7\u201d\u7248ART\u7597\u6cd5\uff08\u5373LASER ART\uff09\uff0cCRISPR-Cas9\u7597\u6cd5\u4ee5\u53ca\u4e24\u8005\u7684\u7ec4\u5408\u7248\u3002<\/p>\n<p>LASER ART\u548cCRISPR-Cas9\u7ec4\u5408\u7597\u6cd5\u7684\u64cd\u4f5c\u8fc7\u7a0b\u662f\uff0c\u5148\u4f7f\u7528\u56db\u5468\u7684ART\u7597\u6cd5\uff0c\u4e4b\u540e\u7814\u7a76\u4eba\u5458\u5c06Cas9\u86cb\u767d\u5305\u88c5\u5728\u4e00\u79cd\u5b8c\u5168\u72ec\u7acb\u7684\u75c5\u6bd2\u2014\u2014\u817a\u76f8\u5173\u75c5\u6bd2\uff08adeno-associated virus\uff0cAAV\uff09\u5185\uff0c\u4ece\u800c\u5c06CRISPR\u5206\u914d\u5230\u5c0f\u9f20\u4f53\u5185\u3002AAV\u4e0d\u4f1a\u81f4\u75c5\uff0c\u5176\u4f5c\u7528\u5c31\u50cf\u4e00\u4e2a\u8f7d\u4f53\uff0c\u589e\u52a0\u4e86CRISPR\u7684\u5faa\u73af\uff0c\u5e2e\u52a9CRISPR\u53d1\u73b0\u5e76\u5207\u9664\u6240\u6709HIV 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